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A total of 1.1 million reads were obtained from the 100 RAS samples analysed. All samples were subjected for PCR amplification of 16S rDNA sequences for library formations and prepared for Ion Torrent technology, which sequences 250 bp fragments. Prior to bacterial community analysis, the stored samples were exposed to cell lysis and extraction of environmental DNA by commercial kits. The sampling times were indicated by the production cycle number (cycle 2-5) and the week number within the cycle (W). The sampling tools were a ladle for capturing biofilter biofilm carriers, toothbrushes for wall biofilm capture, filters for capture of water microbes and scalpels for skin tissue slicing. Samples for depth profiles from all fish tanks were also collected. Additional samples were taken during outbreaks of fish skin wounds, washing of the plant, UV filtration of the inlet water and from various wall biofilms. Sample material was obtained monthly from the biofilter biofilm carriers, the production water (tank 3), the fish skin (tank 3) and the tank 3 wall biofilm. A marine aquaculture recycling system (RAS) for the production of post-smolt was monitored for microbial community structures during the first year of operation. Interview with Michael McConnohie and Melodee Spevack.Interview with Melodee Spevack at Digipedia.: CS1 maint: multiple names: authors list ( link) ^ " "Digimon Adventure Tri." English Dub Brings Back Joshua Seth".^ a b c "Behind The Voice Actors - Melodee Spevack".^ a b "Interview with Melodee Spevack".
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